Despite Tweety’s high pitched voice and long eyelashes- he is actually a male. Tweety bird has changed shape and size and color through time – but since the very beginning it has been established in numerous episodes that he actually a yellow canary bird.
Tweety Bird originally did not have a long eyelashes and such a ‘adorable’ appearance. When he was first created and introduced to the public in 1942 he was a naked baby bird without feather. When Friz Freleng further developed Tweety in 1945 he gave him a cuter appearance by giving him big eyelashes, yellow feathers and blue eyes.
In the 2000 feature animated film ‘Tweety’s High-Flying adventure’ Tweety Bird plays next to new female friend Awooga. Displayed next to a female, it becomes more obvious that Tweety is in fact a male.